Skip to Main content Skip to Navigation
Journal articles

Concerted action of the MutLβ heterodimer and Mer3 helicase regulates the global extent of meiotic gene conversion

Abstract : Gene conversions resulting from meiotic recombination are critical in shaping genome diversification and evolution. How the extent of gene conversions is regulated is unknown. Here we show that the budding yeast mismatch repair related MutLβ complex, Mlh1-Mlh2, specifically interacts with the conserved meiotic Mer3 helicase, which recruits it to recombination hotspots, independently of mismatch recognition. This recruitment is essential to limit gene conversion tract lengths genome-wide, without affecting crossover formation. Contrary to expectations, Mer3 helicase activity, proposed to extend the displacement loop (D-loop) recombination intermediate, does not influence the length of gene conversion events, revealing non-catalytical roles of Mer3. In addition, both purified Mer3 and MutLβ preferentially recognize D-loops, providing a mechanism for limiting gene conversion in vivo. These findings show that MutLβ is an integral part of a new regulatory step of meiotic recombination, which has implications to prevent rapid allele fixation and hotspot erosion in populations.
Document type :
Journal articles
Complete list of metadata

https://hal-cea.archives-ouvertes.fr/cea-03282661
Contributor : Marie-Claude Marsolier Connect in order to contact the contributor
Submitted on : Monday, July 12, 2021 - 10:40:23 AM
Last modification on : Wednesday, October 20, 2021 - 12:25:05 AM
Long-term archiving on: : Wednesday, October 13, 2021 - 6:22:06 PM

File

Dur1.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Yann Duroc, Rajeev Kumar, Lepakshi Ranjha, Céline Adam, Raphaël Guérois, et al.. Concerted action of the MutLβ heterodimer and Mer3 helicase regulates the global extent of meiotic gene conversion. eLife, eLife Sciences Publication, 2017, 6, pp.e21900. ⟨10.7554/eLife.21900⟩. ⟨cea-03282661⟩

Share

Metrics

Record views

101

Files downloads

89