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The molecular architecture of CenH3-deficient holocentromeres in Lepidoptera is dependent on transcriptional and chromatin dynamics

Abstract : Despite their essentiality for chromosome segregation, centromeres are diverse among eukaryotes and embody two main configurations: mono- and holocentromeres, referring respectively to a localized or unrestricted distribution of centromeric activity. Previous studies revealed that holocentricity in many insects coincides with the loss of the otherwise essential centromere component CenH3 (CENP-A), suggesting a molecular link between the two events. In this study, we leveraged recently-identified centromere components to map and characterize the centromeres of Bombyx mori. This uncovered a robust correlation between centromere profiles and regions of low chromatin dynamics. Transcriptional perturbation experiments showed that low chromatin activity is crucial for centromere formation in B. mori . Our study points to a novel mechanism of centromere formation that occurs in a manner recessive to the chromosome-wide chromatin landscape. Based on similar profiles in additional Lepidoptera, we propose an evolutionarily conserved mechanism that underlies the establishment of holocentromeres through loss of centromere specificity.
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Preprints, Working Papers, ...
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https://hal.archives-ouvertes.fr/hal-03063828
Contributor : Ines Drinnenberg Connect in order to contact the contributor
Submitted on : Wednesday, December 30, 2020 - 10:58:00 AM
Last modification on : Tuesday, October 19, 2021 - 11:00:29 AM
Long-term archiving on: : Wednesday, March 31, 2021 - 6:33:27 PM

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2020.07.09.193375v1.full.pdf
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Aruni Senaratne, Héloïse Muller, Kelsey Fryer, Ines Drinnenberg. The molecular architecture of CenH3-deficient holocentromeres in Lepidoptera is dependent on transcriptional and chromatin dynamics. 2020. ⟨hal-03063828⟩

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