Histological, transcriptomic and in vitro analysis reveal an intrinsic activated state of myogenic precursors in hyperplasic muscle of trout - INRA - Institut national de la recherche agronomique Accéder directement au contenu
Article Dans Une Revue BMC Genomics Année : 2018

Histological, transcriptomic and in vitro analysis reveal an intrinsic activated state of myogenic precursors in hyperplasic muscle of trout

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Background: The dramatic increase in myotomal muscle mass in post-hatching fish is related to their ability tolastingly produce new muscle fibres, a process termed hyperplasia. The molecular and cellular mechanismsunderlying fish muscle hyperplasia largely remain unknown. In this study, we aimed to characterize intrinsicproperties of myogenic cells originating from hyperplasic fish muscle. For this purpose, we compared in situproliferation, in vitro cell behavior and transcriptomic profile of myogenic precursors originating from hyperplasicmuscle of juvenile trout (JT) and from non-hyperplasic muscle of fasted juvenile trout (FJT) and adult trout (AT).Results: For the first time, we showed that myogenic precursors proliferate in hyperplasic muscle from JT as shownby in vivo BrdU labeling. This proliferative rate was very low in AT and FJT muscle. Transcriptiomic analysis revealedthat myogenic cells from FJT and AT displayed close expression profiles with only 64 differentially expressed genes(BH corrected p-val < 0.001). In contrast, 2623 differentially expressed genes were found between myogenic cellsfrom JT and from both FJT and AT. Functional categories related to translation, mitochondrial activity, cell cycle,and myogenic differentiation were inferred from genes up regulated in JT compared to AT and FJT myogenic cells.Conversely, Notch signaling pathway, that signs cell quiescence, was inferred from genes down regulated in JTcompared to FJT and AT. In line with our transcriptomic data, in vitro JT myogenic precursors displayed higherproliferation and differentiation capacities than FJT and AT myogenic precursors.Conclusions: The transcriptomic analysis and examination of cell behavior converge to support the view thatmyogenic cells extracted from hyperplastic muscle of juvenile trout are intrinsically more potent to form myofibresthan myogenic cells extracted from non-hyperplasic muscle. The generation of gene expression profiles inmyogenic cell extracted from muscle of juvenile trout may yield insights into the molecular and cellularmechanisms controlling hyperplasia and provides a useful list of potential molecular markers of hyperplasia.
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hal-01958288 , version 1 (18-07-2019)

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Sabrina Jagot, Nathalie Sabin, Aurélie Le Cam, Jérôme Bugeon, Pierre-Yves Rescan, et al.. Histological, transcriptomic and in vitro analysis reveal an intrinsic activated state of myogenic precursors in hyperplasic muscle of trout. BMC Genomics, 2018, 19 (1), pp.1-11. ⟨10.1186/s12864-018-5248-y⟩. ⟨hal-01958288⟩
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