Skip to Main content Skip to Navigation
Journal articles

The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae

Abstract : Fungal adhesins (Als) or flocculins are family of cell surface proteins that mediate adhesion to diverse biotic and abiotic surfaces. A striking characteristic of Als proteins originally identified in the pathogenic Candida albicans is to form functional amyloids that mediate cis-interaction leading to the formation of adhesin nanodomains and trans-interaction between amyloid sequences of opposing cells. In this report, we show that flocculins encoded by FLO11 in Saccharomyces cerevisiae behave like adhesins in C. albicans. To do so, we show that the formation of nanodomains under an external physical force requires a threshold number of amyloid-forming sequences in the Flo11 protein. Then, using a genome editing approach, we constructed strains expressing variants of the Flo11 protein under the endogenous FLO11 promoter, leading to the demonstration that the loss of amyloid-forming sequences strongly reduces cell-cell interaction but has no effect on either plastic adherence or invasive growth in agar, both phenotypes being dependent on the N-and C-terminal ends of Flo11p. Finally, we show that the location of Flo11 is not altered either by the absence of amyloid-forming sequences or by the removal of the Nor C-terminus of the protein.
Complete list of metadata

https://hal.laas.fr/hal-03434693
Contributor : Etienne Dague Connect in order to contact the contributor
Submitted on : Thursday, November 18, 2021 - 1:50:41 PM
Last modification on : Monday, November 29, 2021 - 3:24:03 PM

File

73_elife_Amyloid.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Clara Bouyx, Marion Schiavone, Marie-Ange Teste, Etienne Dague, Nathalie Sieczkowski, et al.. The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae. eLife, eLife Sciences Publication, 2021, 10, pp.e68592. ⟨10.7554/eLife.68592⟩. ⟨hal-03434693⟩

Share

Metrics

Les métriques sont temporairement indisponibles