This paper addresses the problem of regional stability analysis of a continuous-time plant controlled by a discrete-time dynamic output feedback control law with saturation constraints under aperiodic sampling. To cope with the aperiodic sampling problem, the proposed approach is based on an impulsive system modeling and the use of looped functionals. A generalized sector-based relation is applied to tackle the control saturation effects. From these ingredients, conditions to ensure regional asymptotic stability of the closed-loop system under aperiodic sampling are derived. Based on these conditions, LMI-based optimization problems are proposed to compute estimates of the region of attraction of the closed-loop system or to maximize the bound on the maximal admissible interval between two successive sampling instants, for which the regional stability can be ensured with respect to a given set of admissible states. A numerical example illustrates the application of the results.