Effects of the strain background and autolysis process on the composition and biophysical properties of the cell wall from two different industrial yeasts

Abstract : The Saccharomyces cerevisiae cell surface is endowed with some relevant technological properties, notably antimicrobial and biosorption activities. For these purposes, yeasts are usually processed and packaged in an 'autolysed/dried' formula, which may have some impacts on cell surface properties. In this report, we showed using a combination of biochemical, biophysical and molecular methods that the composition of the cell wall of two wine yeast strains was not altered by the autolysis process. In contrast, this process altered the nanomechanical properties as shown by a 2-to 4-fold increased surface roughness and to a higher adhesion to the atomic force microscope tips of the autolysed cells as compared to live yeast cells. Besides, we found that the two strains harboured differences in biomechanical properties that could be due in part to higher levels of mannan in one of them, and to the fact that the surface of this mannan-enriched strain is decorated with highly adhesive patches forming nanodomains. The presence of these nanodomains could be correlated with the upregulation of flocculin encoding FLO11 as well as to higher expression of few other genes encoding cell wall mannoproteins in this mannan-enriched strain as compared to the other strain.
Document type :
Journal articles
FEMS Yeast Research, Oxford University Press (OUP), 2015, 15 (2), pp.12 〈10.1093/femsyr/fou012〉
Liste complète des métadonnées

Cited literature [4 references]  Display  Hide  Download


https://hal.laas.fr/hal-01553141
Contributor : Etienne Dague <>
Submitted on : Tuesday, December 19, 2017 - 3:32:20 PM
Last modification on : Thursday, January 18, 2018 - 4:58:47 PM

Files

Effects of the strain.pdf
Files produced by the author(s)

Identifiers

Citation

Marion Schiavone, Nathalie Sieczkowski, Mathieu Castex, Etienne Dague, Jean Marie François. Effects of the strain background and autolysis process on the composition and biophysical properties of the cell wall from two different industrial yeasts. FEMS Yeast Research, Oxford University Press (OUP), 2015, 15 (2), pp.12 〈10.1093/femsyr/fou012〉. 〈hal-01553141〉

Share

Metrics

Record views

22

Files downloads

16