Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering.

Tiago Cordeiro 1 Fatima Herranz-Trillo 2, 1 Annika Urbanek 1 Alejandro Estaña 1 Juan Cortés 3 Nathalie Sibille 1 Pau Bernadó 1
1 CBS - Centre de Biochimie Structurale [Montpellier]
2 Department of Drug Design and Pharmacology, University of Copenhagen
3 LAAS-RIS - Équipe Robotique et InteractionS
LAAS - Laboratoire d'analyse et d'architecture des systèmes [Toulouse]
Abstract : Intrinsically Disordered Proteins (IDPs) are fundamental actors of biological processes. Their inherent plasticity facilitates very specialized tasks in cell regulation and signalling, and their malfunction is linked to severe pathologies. Understanding the functional role of disorder requires the structural characterization of IDPs and the complexes they form. Small-angle Scattering of X-rays (SAXS) and Neutrons (SANS) have notably contributed to this structural understanding. In this review we summarize the most relevant developments in the field of SAS studies of disordered proteins. Emphasis is given to ensemble methods and how SAS data can be combined with computational approaches or other biophysical information such as NMR. The unique capabilities of SAS enable its application to extremely challenging disordered systems such as low-complexity regions, amyloidogenic proteins and transient biomolecular complexes. This reinforces the fundamental role of SAS in the structural and dynamic characterization of this elusive family of proteins.
Keywords : Nuclear magnetic resonance Intrinsically disordered proteins Ensemble methods Random Coil Models Low-­‐Complexity Regions Small-­‐Angle X-­‐ray Scattering Small-­‐angle Neutron Scattering Ensemble Methods Protein-­‐Protein Interactions
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Advances in Experimental Medicine and Biology, Kluwer, 2017, pp.PP.107-129. 〈10.1007/978-981-10-6038-0_7〉
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Soumis le : jeudi 14 décembre 2017 - 16:52:33
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Tiago Cordeiro, Fatima Herranz-Trillo, Annika Urbanek, Alejandro Estaña, Juan Cortés, et al.. Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering.. Advances in Experimental Medicine and Biology, Kluwer, 2017, pp.PP.107-129. 〈10.1007/978-981-10-6038-0_7〉. 〈hal-01664316〉

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