Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering. - LAAS - Laboratoire d'Analyse et d'Architecture des Systèmes Accéder directement au contenu
Article Dans Une Revue Advances in Experimental Medicine and Biology Année : 2017

Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering.

Résumé

Intrinsically Disordered Proteins (IDPs) are fundamental actors of biological processes. Their inherent plasticity facilitates very specialized tasks in cell regulation and signalling, and their malfunction is linked to severe pathologies. Understanding the functional role of disorder requires the structural characterization of IDPs and the complexes they form. Small-angle Scattering of X-rays (SAXS) and Neutrons (SANS) have notably contributed to this structural understanding. In this review we summarize the most relevant developments in the field of SAS studies of disordered proteins. Emphasis is given to ensemble methods and how SAS data can be combined with computational approaches or other biophysical information such as NMR. The unique capabilities of SAS enable its application to extremely challenging disordered systems such as low-complexity regions, amyloidogenic proteins and transient biomolecular complexes. This reinforces the fundamental role of SAS in the structural and dynamic characterization of this elusive family of proteins.
Fichier principal
Vignette du fichier
Cordeiro_etal_SAS_IDPs_preprint.pdf (1.15 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01664316 , version 1 (14-12-2017)

Identifiants

Citer

Tiago N Cordeiro, Fatima Herranz-Trillo, Annika N Urbanek, Alejandro N Estaña, Juan Cortés, et al.. Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering.. Advances in Experimental Medicine and Biology, 2017, pp.PP.107-129. ⟨10.1007/978-981-10-6038-0_7⟩. ⟨hal-01664316⟩
500 Consultations
43 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More