LAB-ON-CHIP FOR DNA CONCENTRATION AND SEPARATION WITH A RESOLUTION LENGTH OF 6 BP
Résumé
DNA separation and analysis has advanced over the last years benefiting from microfluidic systems that reduce sample volumes and analysis cost. We recently developed a microfluidic device that separates and analyzes nucleic acids using bidirectional actuation with a hydrodynamic flow and a counter electrophoretic force in viscoelastic polymer solutions. In this paper, we provide an analytical model of DNA transport by combining hydrodynamics and statistical mechanics capable of predicting the device performances as a function of the experimental control parameters; namely the pressure, the voltage and the fluid rheological properties. The model proves to be in quantitative agreement with the experiments. We finally define conditions for optimal DNA separation in terms of microchannel geometry, fluid viscoelastic properties and electro-hydrodynamic actuation that result in a resolution length (RSL) down to 6 bp.
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