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Poster Année : 2017

Rapid Discrimination Of Single-Nucleotide Differences Through The Spatial Analysis Of A Nanofluidic-Embedded Biosensor

Résumé

We demonstrate that the spatial analysis of nanofluidic-embedded biosensors permits the fast and direct discrimination of single-nucleotide difference (SND) within microRNA sequences in a single step interaction. We first show that the spatial hybridization profile depends on the duplex affinity, and we speculate that the affinity constant can be inferred during target injection, which is not possible in local analysis where the dissociation step through rinsing must be conducted. We finally manage to discriminate a GT mismatch on a microRNA sequence.
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Dates et versions

hal-01699274 , version 1 (02-02-2018)

Identifiants

  • HAL Id : hal-01699274 , version 1

Citer

Jean Cacheux, Marie Brut, Aurélien Bancaud, Pierre Cordelier, Thierry Leichle. Rapid Discrimination Of Single-Nucleotide Differences Through The Spatial Analysis Of A Nanofluidic-Embedded Biosensor. MicroTAS, Oct 2017, Savannah, United States. 2017. ⟨hal-01699274⟩
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