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Assembly of live micro-organisms on microstructured PDMS stamps by convective/capillary deposition for AFM bio-experiments

Etienne Dague 1 Eric Jauvert 2 Loïc Laplatine 2 B. Viallet 3 Christophe Thibault 1 Laurence Ressier 3 
1 LAAS-ELIA - Équipe Ingénierie pour les sciences du vivant
LAAS - Laboratoire d'analyse et d'architecture des systèmes
2 LAAS-NBS - Équipe NanoBioSystèmes
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : Immobilization of live micro-organisms on solid substrates is an important prerequisite for atomic force microscopy (AFM) bio-experiments. The method employed must immobilize the cells firmly enough to enable them to withstand the lateral friction forces exerted by the tip during scanning but without denaturing the cell interface. In this work, a generic method for the assembly of living cells on specific areas of substrates is proposed. It consists in assembling the living cells within the patterns of microstructured, functionalized poly-dimethylsiloxane (PDMS) stamps using convective/capillary deposition. This versatile approach is validated by applying it to two systems of foremost importance in biotechnology and medicine: Saccharomyces cerevisiae yeasts and Aspergillus fumigatus fungal spores. We show that this method allows multiplexing AFM nanomechanical measurements by force spectroscopy on S. cerevisiae yeasts and high-resolution AFM imaging of germinated Aspergillus conidia in buffer medium. These two examples clearly demonstrate the immense potential of micro-organism assembly on functionalized, microstructured PDMS stamps by convective/capillary deposition for performing rigorous AFM bio-experiments on living cells.
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https://hal.laas.fr/hal-01767584
Contributor : Christophe Thibault Connect in order to contact the contributor
Submitted on : Monday, April 16, 2018 - 1:07:19 PM
Last modification on : Wednesday, June 1, 2022 - 5:06:43 AM

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Etienne Dague, Eric Jauvert, Loïc Laplatine, B. Viallet, Christophe Thibault, et al.. Assembly of live micro-organisms on microstructured PDMS stamps by convective/capillary deposition for AFM bio-experiments. Nanotechnology, Institute of Physics, 2011, 22 (39), pp.395102. ⟨10.1088/0957-4484/22/39/395102⟩. ⟨hal-01767584⟩

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