Biological functionalization of massively parallel arrays of nanocantilevers using microcontact printing

Christophe Thibault 1 Samuel Guillon 2 Sven Salomon 2 Florent Seichepine 2 Denis Dezest 2 Fabrice Mathieu 3 Aude Bouchier 3 Laurent Mazenq 4 Christophe Vieu 1 Thierry Leichle 2 Liviu Nicu 2
1 LAAS-ELIA - Équipe Ingénierie pour les sciences du vivant
LAAS - Laboratoire d'analyse et d'architecture des systèmes
2 LAAS-NBS - Équipe NanoBioSystèmes
LAAS - Laboratoire d'analyse et d'architecture des systèmes
3 LAAS-I2C - Service Instrumentation Conception Caractérisation
LAAS - Laboratoire d'analyse et d'architecture des systèmes
4 LAAS-TEAM - Service Techniques et Équipements Appliqués à la Microélectronique
LAAS - Laboratoire d'analyse et d'architecture des systèmes
Abstract : In this paper, we present a back-end method for biofunctionalizing a large-scale array of nanocantilevers. Our method relies on the use of a modified microcontact printing process where molecules are delivered onto the fragile structures from the grooves of the stamp while its base sits on the chip, thus providing mechanical stability. We have used this method to print antibodies onto fabricated chips containing up to 105 nanostructures/cm2and the presence of antibodies was validated by fluorescent microscopy. Furthermore, measurement of the nanocantilever resonant frequency shifts provoked by a mean added mass of ∼140 fg/cantilever demonstrated that the cantilevers retained their mechanical integrity. Hence, the method presented here aims at providing an answer to the biofunctionalization of freestanding nanostructures for their use as biosensors
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https://hal.laas.fr/hal-01767603
Contributor : Christophe Thibault <>
Submitted on : Monday, April 16, 2018 - 1:32:25 PM
Last modification on : Friday, January 10, 2020 - 9:10:19 PM

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Christophe Thibault, Samuel Guillon, Sven Salomon, Florent Seichepine, Denis Dezest, et al.. Biological functionalization of massively parallel arrays of nanocantilevers using microcontact printing. Sensors and Actuators B: Chemical, Elsevier, 2012, 161 (1), pp.1135 - 1138. ⟨10.1016/j.snb.2011.10.084⟩. ⟨hal-01767603⟩

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