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Article Dans Une Revue Microelectronic Engineering Année : 2019

Fabrication of 3D microdevices from planar electroplating for the isolation of cancer associated cells in blood

Résumé

Malignant cells detaching from a primary tumor to reach the bloodstream are known as circulating tumor cells (CTCs). Enumeration and molecular characterizations of these cells have been used as prognostic cancer biomarkers. Various in vitro techniques are available to extract these biomarkers from blood samples, although their performances are limited by the amount of blood available for analysis, usually 7.5 mL, and the rareness of these cells as a few per mL. To address this sampling bias we propose a three-dimensional (3D) microdevice adapted to the in vivo isolation of CTCs in the venous blood flow. Our device acts as an in vivo microfilter in which CTCs are specifically retained due to their specific morphological and mechanical properties as compared to normal blood cells. We present the fabrication process of these devices using planar silicon technology and their adaptation to generate a 3D microfilter integrated into a compatible medical consumable for venous access. We demonstrate that these devices are capable to capture human prostate cancer cells (PC3) spiked into whole blood using a fluidic platform mimicking an artificial vein. Moreover, the captured cells can be easily characterized by conventional microscopy and readily available for functional and downstream analysis. This minimally invasive technology could offer high-quality information to physicians and serve as a tool for personalized therapeutic follow-up in clinical routine.
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Dates et versions

hal-02336950 , version 1 (29-10-2019)

Identifiants

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Elise Bou, Alejandro Kayum Jimenez Zenteno, Aurore Estève, David Bourrier, Christophe Vieu, et al.. Fabrication of 3D microdevices from planar electroplating for the isolation of cancer associated cells in blood. Microelectronic Engineering, 2019, 213, pp.69-76. ⟨10.1016/j.mee.2019.04.010⟩. ⟨hal-02336950⟩
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