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Journal articles
Flanking regions determine the structure of the poly-glutamine homo- repeat in huntingtin through mechanisms common among glutamine-rich human proteins
Abstract : The causative agent of Huntington's disease, the poly-Q homo-repeat in the N-terminal region of huntingtin (httex1), is flanked by a 17-residue-long fragment (N17) and a proline-rich region (PRR), which promote and inhibit the aggregation propensity of the protein, respectively, by poorly understood mechanisms. Based on experimental data obtained from site-specifically labeled NMR samples, we derived an ensemble model of httex1 that identified both flanking regions as opposing poly-Q secondary structure promoters. While N17 triggers helicity through a promiscuous hydrogen bond network involving the side chains of the first glutamines in the poly-Q tract, the PRR promotes extended conformations in neighboring glutamines. Furthermore, a bioinformatics analysis of the human proteome showed that these structural traits are present in many human glutamine-rich proteins and that they are more prevalent in proteins with longer poly-Q tracts. Taken together, these observations provide the structural bases to understand previous biophysical and functional data on httex1.
https://hal.laas.fr/hal-02893075
Contributor : Juan Cortés <>
Submitted on : Wednesday, July 8, 2020 - 8:12:27 AM
Last modification on : Wednesday, June 30, 2021 - 4:06:02 PM
Long-term archiving on: : Monday, November 30, 2020 - 1:19:37 PM
Contributor : Juan Cortés <>
Submitted on : Wednesday, July 8, 2020 - 8:12:27 AM
Last modification on : Wednesday, June 30, 2021 - 4:06:02 PM
Long-term archiving on: : Monday, November 30, 2020 - 1:19:37 PM