Skip to Main content Skip to Navigation
New interface
Journal articles

Atomic force microscopy-single-molecule force spectroscopy unveils GPCR cell surface architecture

Abstract : G protein-coupled receptors (GPCRs) form the largest family of cell surface receptors. Despite considerable insights into their pharmacology, the GPCR architecture at the cell surface still remains largely unexplored. Herein, we present the specific unfolding of different GPCRs at the surface of living mammalian cells by atomic force microscopy-based single molecule force spectroscopy (AFM-SMFS). Mathematical analysis of the GPCR unfolding distances at resting state revealed the presence of different receptor populations relying on distinct oligomeric states which are receptor-specific and receptor expression-dependent. Moreover, we show that the oligomer size dictates the receptor spatial organization with nanoclusters of high-order oligomers while lower-order complexes spread over the whole cell surface. Finally, the receptor activity reshapes both the oligomeric populations and their spatial arrangement. These results add an additional level of complexity to the GPCR pharmacology until now considered to arise from a single receptor population at the cell surface. Atomic force microscopy-based single molecule force spectroscopy reveals the unfolding of G-protein coupled receptors on the surface of living mammalian cells.
Document type :
Journal articles
Complete list of metadata

https://hal.inrae.fr/hal-03619367
Contributor : Suzette Astruc Connect in order to contact the contributor
Submitted on : Friday, March 25, 2022 - 9:35:11 AM
Last modification on : Wednesday, November 23, 2022 - 12:00:23 PM
Long-term archiving on: : Sunday, June 26, 2022 - 6:20:41 PM

File

2022_Dague_Communications-Biol...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Etienne Dague, Véronique Pons, Alexandre Roland, Jean-Marc Azaïs, Silvia Arcucci, et al.. Atomic force microscopy-single-molecule force spectroscopy unveils GPCR cell surface architecture. Communications Biology, 2022, 5 (1), ⟨10.1038/s42003-022-03162-w⟩. ⟨hal-03619367⟩

Share

Metrics

Record views

112

Files downloads

29