Conception et caractérisation d'un dispositif à base de nanopores destiné à l'enregistrement électrique de l'activité de canaux ioniques membranaires

Raphaël Marchand 1
1 LAAS-ELIA - Équipe Ingénierie pour les sciences du vivant
LAAS - Laboratoire d'analyse et d'architecture des systèmes [Toulouse]
Abstract : Ion channels are membrane proteins responsible for ion transport across biological membranes. Due to their ubiquity, they are promising drug targets but are not yet fully exploited as such due to experimental restrictions in their study. Electrical measurement of ion channels activity within in vitro artificial lipid bilayers would enable to overcome these restrictions. However, there is not yet a system satisfying all the requirements for ion channels studies: stability and purity of the lipid bilayer, low noise level, fast insertion of ion channels, fluidic integration, ability to perform simultaneous optical characterization. The aim of this phD was to assess in which extent the use of an SOI (Silicon On Insulator) substrate bearing nanopores could satisfy all these requirements. 10 nm to 160 nm diameter nanopores were fabricated in an SOI substrate and characterized. A transparent fluidic cell was used for fluidic addressing. This transparent cell allows combined electrical and optical characterization. Electrical properties of the device in aqueous environment were studied, allowing to bring out improvement prospects. The combined electrical and optical characterization was demonstrated with fluorescent nanoparticle trapping experiments on the nanopores. Finally, promising results about the formation of a free-standing lipid bilayer are presented.
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Micro et nanotechnologies/Microélectronique. Université Paul Sabatier - Toulouse III, 2016. Français. 〈NNT : 2016TOU30229〉
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Raphaël Marchand. Conception et caractérisation d'un dispositif à base de nanopores destiné à l'enregistrement électrique de l'activité de canaux ioniques membranaires. Micro et nanotechnologies/Microélectronique. Université Paul Sabatier - Toulouse III, 2016. Français. 〈NNT : 2016TOU30229〉. 〈tel-01416500v2〉

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