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Intégration et optimisation de procédés de séparation d'ADN

Abstract : This manuscript describes and discusses the integration of instrumentation hardware for the µLAS system, for the optimization of the analysis and separation of DNA molecules. First, we will discuss separation techniques, their approaches and strategies. And we will try to answer this question which is asked when a species migrates: how to characterize the competition between forced advection and natural diffusion? To optimize and improve the performance of the various separation technologies thus mentioned, it is necessary to introduce the issues inherent in each. Because there is indeed a wide range of actuation means that cause molecules to migrate in multiple ways: by hydrodynamics, by electrophoresis or even techniques combining the two approaches. Then we will introduce the µLAS technology and in what technological context it is placed. We will present the physical principles that govern the different stages of the technology's operation (concentration and separation). We will then approach the developments carried out during this thesis work on a temporal separation mode which involves a new chip model. Finally, we will show the development work, both in technological manufacturing and also in software intelligence, to set up slope engravings in silicon and thus enrich the µLAS system. Then we will introduce the instrumentation work carried out throughout these 3 years on a dedicated experimental bench. This chapter will be organized to introduce an uninformed reader to the basics and issues inherent in programming, instrumentation and automation of systems controlled by LabVIEW. We will use these bases to present the platform thus developed to pilot µLAS experiments. Finally, such a system needs to be characterized because it involves many players: computer and mechanical tools. Finally, we will present the separation results obtained using the fully integrated and automated µLAS platform. The analysis of these results raised questions and at the same time a study of the dynamic dispersion of DNA bands. We will end with some prospects for improvement, mentioned during the manuscript.
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Submitted on : Tuesday, May 18, 2021 - 12:20:30 PM
Last modification on : Wednesday, June 9, 2021 - 10:00:33 AM


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  • HAL Id : tel-03228680, version 2


Jeffrey Teillet-Deborde. Intégration et optimisation de procédés de séparation d'ADN. Physique [physics]. Université Paul Sabatier - Toulouse III, 2020. Français. ⟨NNT : 2020TOU30127⟩. ⟨tel-03228680v2⟩



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