Comparative study of fully three-dimensional reconstruction algorithms for lens-free microscopy

Abstract : We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multiangle acquisitions on 3D cell cultures embedded in extracellular matrices. Lens-free microscopy acquisitions present some inherent issues such as the lack of phase information on the sensor plane and a limited angular coverage. We developed and compared three different algorithms based on the Fourier diffraction theorem to obtain fully 3D reconstructions. These algorithms present an increasing complexity associated with a better reconstruction quality. Two of them are based on a regularized inverse problem approach. To compare the reconstruction methods in terms of artefact reduction, signal-to-noise ratio, and computation time, we tested them on two experimental datasets: an endothelial cell culture and a prostate cell culture grown in a 3D extracellular matrix with large reconstructed volumes up to ∼5  mm3∼5  mm3 with a resolution sufficient to resolve isolated single cells. The lens-free reconstructions compare well with standard microscopy.
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Submitted on : Thursday, August 22, 2019 - 4:19:34 PM
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Anthony Berdeu, Fabien Momey, Bastien Laperrousaz, Thomas Bordy, Xavier Gidrol, et al.. Comparative study of fully three-dimensional reconstruction algorithms for lens-free microscopy. Applied optics, Optical Society of America, 2017, 56 (13), pp.3939-3951. ⟨10.1364/AO.56.003939⟩. ⟨hal-01561175⟩



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