Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth

Abstract : 3D fabrication techniques are rapidly expanding in the field of scaffold development for cell culture and tissue engineering. Herein we report the realization of free-standing PEGDA hydrogel architectures by using two-photon lithography. The morphological and immunofluorescence characterization of neuro2A cells revealed a tridimensional colonization featuring multiple neuritic extensions per cell as well as the expression of β-tubulin neuronal marker and actin microfilaments. The results open new perspectives in the continuous quest for structured biomaterials able to provide a favorable environment to cells and at the same time not interfering with imaging protocols necessary for a clear scenario of the cell seeding.
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https://hal.laas.fr/hal-01698486
Contributor : Angelo Accardo <>
Submitted on : Thursday, February 1, 2018 - 12:59:16 PM
Last modification on : Friday, January 10, 2020 - 9:10:19 PM

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Angelo Accardo, Marie-Charline Blatché, Rémi Courson, Isabelle Loubinoux, Christophe Vieu, et al.. Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth. Biomedical Physics & Engineering Express, IOP Publishing, 2018, 4 (2), pp.027009. ⟨10.1088/2057-1976/aaab93⟩. ⟨hal-01698486⟩

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