The main objective of this study was to investigate the effect of mixed cooling techniques (combination of internal and external strategies, with and without menthol) during warm-up for a time trial in tropical climate. Seven heat-acclimatized trained male road cyclists participated in three experimental sessions consisting of 20-min cycling performances on a velodrome track in ecological hot and humid conditions (Guadeloupe, French West Indies; WBGT: 27.64±0.27°C; relative humidity: 76.43±2.19%), preceded by a standardized 30-min warm-up and the ingestion of cold menthol water (1) with a cooling vest soaked in ice water (ICE-VEST), (2) with a cooling vest soaked in ice menthol water (MEN-VEST), and (3) without a vest (NO-VEST). Cycling performance (total distance, distance traveled per 2-min block), physiological parameters (core body temperature recorded, heart rate) and perceptions (exertion, thermal comfort, thermal sensation) were assessed. No between-condition differences were found for physiological parameters, the total covered distance or the distance traveled per 2-min block. However, distance traveled per 2-min decreased with time (p = 0 . 03) , with no difference between conditions, suggesting a variation in pace during the cycling performance trial (e.g., mean±SD: 1321±48.01m at T2; 1308±46.20m at T8, 1284±78.38m at T14, 1309±76.29m at T20). No between-condition differences were found for perception of exertion, thermal comfort and thermal sensation during the warm-up (11.83±3.34; 2.58±1.02; 4.39±0.94, respectively) and the performance (17.85±0.99; 2.70±1.25; 5.20±1.20, respectively) but the pairwise comparisons within condition revealed a significant increase of TS values from T0 (4.57±1.13) to T20 (6.00±0.58) only in NO-VEST condition ( p = 0.04). The absence of modification of thermal sensation at the end of the cycling test under the mixed conditions (ICE-VEST and MEN-VEST) suggests a beneficial effect of wearing a cooling vest on thermal sensation although it had no effect on performance.